The functional elements of the vector are the pUC origin of replication (pUC ori) for propagation in E.coli, the matrix attached region from the 5′-region of the human Interferone β-gene (S/MAR), the promoter of the bacterial ampicillin resistence gene for selection in E.coli (pAmp), the SV40 early promoter for selection in CHO cells (pSV40), the SV40 origin of replication (SV40 ori), the kanamycin resistance gene from Tn5 from E.coli for selection in E.coli and CHO cells (Kan/Neo) pUC19. Enzymes with unique restriction sites are shown in bold type Analyze Sequence ori = origin of replication Ap = ampicillin References (1) Yanisch-Perron, C., Vieira, J. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present. Durch knockout von ROP kann die Kopienzahl von ColEI Plasmiden generell erhöht werden, solange die Kultivierungstemperatur bei >30 ☌ liegt Full Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Der ori wurde verkürzt und ihm fehlt die codierende Region für das ROM/ROP Protein. Die pUC-Plasmide benutzen den modifizierten origin of replication oder kurz ori des pBR322-Plasmids.
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